from NITE (Japan)
cDNA sequence:
acagacactgatctcagtttcccccgagagtgtgagtgtaggcctctaca
ggcagctgcctctggggagttggggagcctaggggttaactctagacccc
cctcaaccctctggaccaaagtctgtccaggaaaacaggatgccggagcc
caggagtcgtcagcctagcagttgcctggcctccagatgcctcccagggt
caccttccagccctgtggatggcagtggaatcaggacactcccttgaaca
gtgaatacgattttgccctggtcaacattggacgattagaggctgtgagc
ggcttgtcccgagtccagctcctccgtccagggtccctgcataaatttat
ccctgaggagattctgattcatggccgagacttccggctgctcagagttg
gttttgaggctggaggcctagagcctcaggcttttcaggtgaccatggcc
attgtccaagccagagctcagagcaatcaagcccaacagtattcggggat
aaccctgagcaaggctggccagggttctggctccagaaaaccaccaattc
ctctcatggagacagcggaagactgggagactgagcggaagaagcaggca
gccagaggctggagggtcagcacggtcaacgagaggttcgacgtagccac
cagcctcccccgttacttctgggtccctaaccgaattctggacagtgagg
tcaggagagcatttggccactttcatcagggccgtggaccgcgcttgtcc
tggcatcgccctgggggcagtgatcttctccgctgtggaggcttctatac
agccagtgaccctaacaaggaggatatcagagcagtggagttgatgctcc
aggctgggcattcagatgttgtcctggtagacactatggatgagctgccc
agccttgcagatgtccaacttgcccacctgaggctgagggccctctgcct
gcctgattcatctgtagctgaggataaatggctttcagccctggaaggaa
cacgatggctggactatgtcagggcttgtcttcgaaaggccagtgacatt
tcagtattagtgacatccagggttcgttctgtaatacttcaagagcgcgg
tgatcgtgatctcaatggcctcctctcttcactcgtccagctgctttcag
cccccgaagcccgaacactgtttggcttccaatcactagtacagcgagag
tgggtggcagctggacatcccttcctgactcggcttgggggaactggggc
cagtgaagaggctccggtgtttctcctcttccttgattgtgtctggcagc
tcctccagcagtttccagctgattttgaattctctgagtttttccttctt
gctcttcatgacagtgtcagggttcctgacacccttaccttcctgagaaa
taccccctgggagcgcggaaagcagagcggacagttaaactcctatacac
aagtctacaccccaggatactcccagcctccagctgggaactcttttaac
ctgcagctgtctgtctgggactgggatttacgttatagcaatgcacagat
actacaattccagaatcctggctatgacccagaacactgtccagattcct
ggctccctagaccacagccaagcttcatggttcctggaccccccagttct
gtgtggctcttctctagaggagcattgacccccctgaatcagctctgtcc
ttggcgggacagtccttccctgctggcagtctcttctcgttggctccctc
gacctgctatctcctctgaaagcctggctgaccaggaatggggtctcccc
tcacattggggagcttgccctttacctccagggctgctgctgcctgggta
tctgggaccccagatcaggctctggagacgctgctacctgaggggaaggc
ctgaggtccagatgggcctctcagctcccacaatctctggcctccaggat
gagctatcccatcttcaggagttattacggaaatggacaccaagaatatc
tcctgaggatcactccaagaaaagagatccacataccattctcaatccca
ctgaaattgctggcattctcaaaggccaatcccatcccttctggataacc
agatgttaaatcatgagatcagagatgctgttcatcagtcccaacaagat
ggcctagaaatcgcattctcacctcgccttgctgctgctttaattccaag
ttctatttcttcccttatagttttctatgggaatgaggcggatacaggaa
acaccctatctcctctgtatttttgtagtggaatttctatttaaggggct
cattaaagcatagtatttatacac
Protein Sequence (640 aa):
MPPRVTFQPCGWQWNQDTPLNSEYDFALVNIGRLEAVSGLSRVQLLRPGS
LHKFIPEEILIHGRDFRLLRVGFEAGGLEPQAFQVTMAIVQARAQSNQAQ
QYSGITLSKAGQGSGSRKPPIPLMETAEDWETERKKQAARGWRVSTVNER
FDVATSLPRYFWVPNRILDSEVRRAFGHFHQGRGPRLSWHRPGGSDLLRC
GGFYTASDPNKEDIRAVELMLQAGHSDVVLVDTMDELPSLADVQLAHLRL
RALCLPDSSVAEDKWLSALEGTRWLDYVRACLRKASDISVLVTSRVRSVI
LQERGDRDLNGLLSSLVQLLSAPEARTLFGFQSLVQREWVAAGHPFLTRL
GGTGASEEAPVFLLFLDCVWQLLQQFPADFEFSEFFLLALHDSVRVPDTL
TFLRNTPWERGKQSGQLNSYTQVYTPGYSQPPAGNSFNLQLSVWDWDLRY
SNAQILQFQNPGYDPEHCPDSWLPRPQPSFMVPGPPSSVWLFSRGALTPL
NQLCPWRDSPSLLAVSSRWLPRPAISSESLADQEWGLPSHWGACPLPPGL
LLPGYLGPQIRLWRRCYLRGRPEVQMGLSAPTISGLQDELSHLQELLRKW
TPRISPEDHSKKRDPHTILNPTEIAGILKGQSHPFWITRC
TPRISPEDHSKKRDPHTILNPTEIAGILKGQSHPFWITRC
SUBCLONING STRATEGY FOR YEAST TWO-HYBRID SCREENING AND CO-IP/CO-IF